2004年,哈弗医学院的Jonathan Tilly及其同事在老鼠卵巢里发现了干细胞。迄今已经证实,此类卵巢干细胞能够分化成为卵细胞,卵细胞在受精后还能发育成健康的鼠宝宝。但是研究者尚不能确信人类也存在类似机能。
Proving that this was indeed the case was tricky. Human ovarian tissue-especially fromyoung, healthy donors-is not easy to come by. Dr Tilly s breakthrough came when hediscovered that a former colleague, Yasushi Takai of Saitama Medical University in Japan,had in his freezer healthy ovarian tissue from 30 patients who had changed sex.
想要弄清真相有些困难。人类卵巢组织很难找到,健康的年轻捐献者的组织更难寻觅。幸而Tilly博士发现他的前同事日本埼玉医科大学的Yasushi Takai在冰箱里存放了30个变性人不再需要的健康卵巢组织,取得了突破。
Using a sophisticated cell-sorting technique, the researchers developed a way to identifyovarian stem cells that works for both mice and humans. Then they took the human ovarianstem cells, labelled them with a green fluorescent protein and put them back into a slice ofhuman ovary . The glowing green cells soon produced a brand new crop of human eggs, accordingto their findings published this week in Nature Medicine.
研究人员运用尖端的细胞分选技术,找出方法,证实人类和老鼠身上都存在卵巢干细胞。随后,他们分离出人类卵巢干细胞,用荧光蛋白做上标记再把它植入一片人类卵巢组织。这片组织被移植到了活鼠体内,使其保持正常卵巢的机能。他们本周发表在《自然医学》上的结果称,这些绿芒闪烁的细胞很快制造出了一批人类卵细胞。
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