The laboratory's results received the backing of the United States Anti-Doping Agency (USADA), which had challenged Landis at his California arbitration hearing earlier this year.
"We expect the same result that we had the first time around," USADA attorney Richard Young told Reuters. "But it's a fair system and that's his right."
Landis is the first rider in the Tour's history to be stripped of the title for a doping offence.
"We have always believed in the evidence showing that the French laboratory's flawed techniques and conclusions resulted in a false positive result," Maurice Suh, Landis's lawyer, said in a statement to Reuters.
"This appeal is directed at having a fair-minded arbitration panel recognize those errors, and apply the facts and law to this case.
"If this is done, Floyd will have the justice that he seeks."
Oscar Pereiro of Spain, the 2006 Tour runner-up, will receive the winner's yellow jersey in a ceremony on Monday in Madrid.
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