Two-time champion Serena Williams advanced with a comfortable 6-2, 6-1 win over Jarmila Groth.
Sharapova walked on to Centre Court dressed to impress but her tennis looked decidedly second-hand as she suffered a terrible start against the sultry Dulko, dropping the first set in 35 minutes.
The Florida-based Russian, who returned from a nine-month absence from the tour in May following her shoulder problems, showed her characteristic fighting qualities to take the second set and looked favorite to win through.
However, Dulko responded in a thrilling decider and, despite spurning her first four match points, she held her nerve to make Sharapova the highest-profile casualty of the tournament so far.
(Source: Shanghai Daily/Agencies)
Zheng Jie of China reacts during the women's singles second round match against Daniela Hantuchova of Slovakia at the 2009 Wimbledon Tennis Championships in London on June 24, 2009.
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