Over the years, biologists have suggested two main pathways by which sexual selection ) may have shaped the evolution of male birdsong. In the first, male competition and intrasexual selection produce relatively short, simple songs used mainly in territorial behavior. In the second, female choice and intersexual selection produce longer, more complicated songs used mainly in mate attraction; like such visual ornamentation as the peacocks tail, elaborate vocal characteristics increase the males chances of being chosen as a mate, and he thus enjoys more reproductive success than his less ostentatious rivals. The two pathways are not mutually exclusive, and we can expect to find examples that reflect their interaction. Teasing for microscopic examination) them apart has been an important challenge to evolutionary biologists.
Early research confirmed the role of intrasexual selection. In a variety of experiments in the field, males responded aggressively to recorded songs by exhibiting territorial behavior near the speakers. The breakthrough for research into intersexual selection came in the development of a new technique for investigating female response in the laboratory. When female cowbirds raised in isolation in sound-proof chambers were exposed to recordings of male song, they responded by exhibiting mating behavior. By quantifying the responses, researchers were able to determine what particular features of the song were most important. In further experiments on song sparrows that is brownish above and white below with brownish streaks on the breast and that is noted for it melodious song), researchers found that when exposed to a single song type repeated several times or to a repertoire of different song types, females responded more to the latter. The beauty of the experimental design is that it effectively rules out confounding variables; acoustic isolation assures that the female can respond only to the song structure itself.
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