然后将这些肝脏内胚层细胞与其他两种细胞一起培养。一种是取自脐带的内皮细胞,这种细胞构成了血管内层;另一种细胞是取自骨髓的间充质干细胞,这种细胞可以分化成多种细胞类型。
Cultures without mesenchymal stem cells failed to form a cluster.
实验证明,如果培养过程中缺少间充质干细胞,就不会形成簇集物;
Those without endothelial cells failed to create a network of blood vessels.
而如果没有内皮细胞,就无法形成血管网络。
But together, the three types of cell, with little additional prodding, formed a bud withintwo days.
只有将肝脏内胚层细胞与这两者结合,并配合少量的其他刺激,才最终能在两天内形成肝芽。
At six days this bud was expressing genes known to be early markers of the liver.
到了第六天,肝芽开始表达一些在肝脏早期形成阶段具有标志性的基因。
And when Dr Takebe implanted such buds into the brains of mice whose immune systemshad been disabled to prevent rejection, he observed that they connected with the mouse sblood system within two days.
随后,武部博士将这些肝芽结构植入一些丧失免疫能力的老鼠的脑中,这样老鼠就不会对肝芽组织形成排斥反应。武部博士发现,两天内肝芽结构就与这些老鼠脑中的血液循环系统相互融合了。
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