"A major aspiration in the molecular life sciences is the ability to precisely make any change to the genome in any location," says genome biologist David Liu from the the Broad Institute.
布罗德研究所的基因组生物学家David Liu说:“分子生命科学的一个主要目标是能精确改变任何位置的基因组。”
"We're not aware of another editing technology in mammalian cells that offers this level of versatility and precision with so few byproducts."
“我们不知道有其他对哺乳动物细胞进行编辑的技术能像这一技术一样用途广泛、精度高,而且副作用这么少。”
The basis of the new prime editing method is an enzyme called reverse transcriptase. The CRISPR system also uses an enzyme – Cas9 – to cut DNA strands, so that alternative genetic code can be inserted.
这一新的先导编辑方法的基础是被称为逆转录酶的酶,CRISPR系统也利用这种Cas9酶切割DNA链,插入替代遗传密码。
Now, thanks to reverse transcriptase, which is used in conjunction with Cas9 in prime editing, genomic editing has been upgraded again.
现在多亏有了逆转录酶,基因组编辑再次升级了。逆转录酶与Cas9一起被用于先导编辑。
In prime editing, a guide RNA called pegRNA guides a modified form of the Cas9 enzyme to snip only a single strand of DNA (preventing the double-strand breaks that can induce unintended disruptions).
【新的基因编辑技术诞生,更精准,风险更小】相关文章:
最新
2020-09-15
2020-09-15
2020-09-15
2020-09-15
2020-09-15
2020-09-15